fosb antibody Search Results


90
R&D Systems fosb
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Fosb, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fosb/product/R&D Systems
Average 90 stars, based on 1 article reviews
fosb - by Bioz Stars, 2026-03
90/100 stars
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95
Cell Signaling Technology Inc anti fosb antibody
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Anti Fosb Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti fosb antibody/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
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90
Active Motif fosb (#39022)
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Fosb (#39022), supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Atlas Antibodies fosb antibodies
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Fosb Antibodies, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio rabbit anti fos monoclonal antibody
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Rabbit Anti Fos Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
rabbit anti fos monoclonal antibody - by Bioz Stars, 2026-03
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91
Cusabio rabbit polyclonal anti fosb antibody
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Rabbit Polyclonal Anti Fosb Antibody, supplied by Cusabio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Assay Biotechnology phospho-ser27-fosb
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Phospho Ser27 Fosb, supplied by Assay Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho-ser27-fosb/product/Assay Biotechnology
Average 90 stars, based on 1 article reviews
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Geneka Biotechnology Inc antibodies against c-jun, junb, jund, c-fos, and fosb
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Antibodies Against C Jun, Junb, Jund, C Fos, And Fosb, supplied by Geneka Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against c-jun, junb, jund, c-fos, and fosb/product/Geneka Biotechnology Inc
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antibodies against c-jun, junb, jund, c-fos, and fosb - by Bioz Stars, 2026-03
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ABclonal Biotechnology fosb
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Fosb, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fosb/product/ABclonal Biotechnology
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Federation of European Neuroscience Societies pan-fosb antibody sc-48
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Pan Fosb Antibody Sc 48, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pan-fosb antibody sc-48/product/Federation of European Neuroscience Societies
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Beyotime fosb antibody
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Fosb Antibody, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Neuros Medical Inc anti-fosb antibody
Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against <t>MAP3K7,</t> <t>ARHGEF18,</t> <t>FOSB.</t> Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.
Anti Fosb Antibody, supplied by Neuros Medical Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-fosb antibody/product/Neuros Medical Inc
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anti-fosb antibody - by Bioz Stars, 2026-03
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Image Search Results


Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against MAP3K7, ARHGEF18, FOSB. Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.

Journal: Molecular Systems Biology

Article Title: Identification of covalent modifications regulating immune signaling complex composition and phenotype

doi: 10.15252/msb.202010125

Figure Lengend Snippet: Overview of the dynamic and static PPIs and PTMs identified in 17 different cell lines. Global PPI and PTM differences upon PAM3CSK4 activation between prey proteins, and acetylated, methylated, and phosphorylated peptides. Comparison of TRAF2 interactors in native (0.05% NP‐40 in lysis buffer) versus denaturing (6 M GdmCl in lysis buffer). Intensity profile of TRAF2 interactors in different TRAF2 phospho‐variants, normalized to TRAF2 wild‐type intensities. Differential expression of ISG15 in K‐>R TRAF2 mutants versus wild‐type TRAF2 in full proteomes and interactomes. Central band of the boxplot shows the median, boxes represent the IQR, and 2 biological replicates were performed per condition. Analytical size‐exclusion chromatography profile of TRAF2‐ISG15 binding studies. For each analysis, the individual profiles are shown and indicated by color. Coomassie‐stained SDS–PAGE lanes correlate with the approximate retention times in the chromatogram. Western blot analysis of CRISPR‐KO reporter cell lines with antibodies raised against MAP3K7, ARHGEF18, FOSB. Tubulin was used as a loading control. Heatmap of MAP3K7 interactors after treatment with different MAPK14 inhibitors, with significant hits in at least one treatment ( t ‐test, P –value < 0.05) denoted with an asterisk. Treatments were normalized to DMSO control. Heatmap of TRAF2 interactors after treatment with different MAPK14 inhibitors. Treatments were normalized to DMSO control. Intensity profile of MAP3K7 phosphorylation on Ser367, Ser412, and Ser445 after treatment with different MAPK14 inhibitors, normalized to MAP3K7 bait intensity. Central band of the boxplot shows the median, boxes represent the IQR, and four biological replicates were used per condition. P ‐values were calculated by t ‐test. Asterisks indicate significant differences **** P ‐value < 0.0001, * P ‐value < 0.05, *** P ‐value < 0.001.

Article Snippet: Antibodies used for immunoblotting were as follows (diluted 1:1,000): phospho‐p38 MAPK (Thr180/Tyr182) antibody (Cell Signaling, 9211), GAPDH (14C10) rabbit mAb (Cell Signalling, 2118), p38 MAPK (R&D, AF8691), ARHGEF18 (Sigma, HPA042689), MAP3K7 (R&D, MAB5307), FOSB (R&D, AF2214) and anti‐rabbit IgG, HRP‐linked antibody (Cell Signaling, 7074).

Techniques: Activation Assay, Methylation, Lysis, Expressing, Size-exclusion Chromatography, Binding Assay, Staining, SDS Page, Western Blot, CRISPR

Journal: Molecular Systems Biology

Article Title: Identification of covalent modifications regulating immune signaling complex composition and phenotype

doi: 10.15252/msb.202010125

Figure Lengend Snippet:

Article Snippet: Antibodies used for immunoblotting were as follows (diluted 1:1,000): phospho‐p38 MAPK (Thr180/Tyr182) antibody (Cell Signaling, 9211), GAPDH (14C10) rabbit mAb (Cell Signalling, 2118), p38 MAPK (R&D, AF8691), ARHGEF18 (Sigma, HPA042689), MAP3K7 (R&D, MAB5307), FOSB (R&D, AF2214) and anti‐rabbit IgG, HRP‐linked antibody (Cell Signaling, 7074).

Techniques: Recombinant, CRISPR, Plasmid Preparation, Clone Assay, Protease Inhibitor, Lysis, Transfection, Reporter Assay, Mutagenesis, Software